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Updated: Feb 8, 2026

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
Published on: August 9, 2019
Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting
J Alejandro Perdomo1, Cristina R G Sales2, Elizabete Carmo-Silva3
1Plant Science Department, Rothamsted Research, Harpenden, UK.
Abstract:
In this chapter, we describe a method to extract and quantify photosynthetic enzymes using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The method is particularly suitable for characterizing altered protein amounts in leaves of plants produced from genetic engineering or gene-editing approaches. We focus on RuBisCO and RuBisCO activase, a molecular chaperone required to sustain the activity of RuBisCO and CO2 fixation, yet the method can be easily adapted to investigate other leaf proteins of interest.
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