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Updated: Feb 8, 2026

Production and Detection of Reactive Oxygen Species ROS in Cancers
Published on: November 21, 2011
Downstream Simultaneous Electrochemical Detection of Primary Reactive Oxygen and Nitrogen Species Released by Cell
Yun Li1, Catherine Sella1, Frédéric Lemaître1
1PASTEUR, Département de chimie , École normale supérieure, PSL Université, Sorbonne Université, CNRS , 75005 Paris , France.
Abstract:
An innovative microfluidic platform was designed to monitor electrochemically four primary reactive oxygen (ROS) and reactive nitrogen species (RNS) released by aerobic cells. Taking advantage of the space confinement and electrode performances under flow conditions, only a few experiments were sufficient to directly provide significant statistical data relative to the average behavior of cells during oxidative-stress bursts. The microfluidic platform comprised an upstream microchamber for cell culture and four parallel microchannels located downstream for separately detecting H2O2, ONOO-, NO·, and NO2-. Amperometric measurements were performed at highly sensitive Pt-black electrodes implemented in the microchannels. RAW 264.7 macrophage secretions triggered by a calcium ionophore were used as a way to assess the performance, sensitivity, and specificity of the integrated microfluidic device. In comparison with some previous evaluations achieved from single-cell measurements, reproducible and relevant determinations validated the proof of concept of this microfluidic platform for analyzing statistically significant oxidative-stress responses of various cell types.
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