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HappyTools: A software for high-throughput HPLC data processing and quantitation.

Bas Cornelis Jansen1, Lise Hafkenscheid2, Albert Bondt2,3

  • 1Research & Development, Ludger Limited., Abingdon, Oxfordshire, United Kingdom.

Plos One
|July 7, 2018
PubMed
Summary
This summary is machine-generated.

HappyTools enhances high-performance liquid chromatography (HPLC) analysis for large studies. This new toolkit offers superior precision and throughput for quantitation, improving data analysis efficiency.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Pharmacology

Background:

  • High-performance liquid chromatography (HPLC) is crucial for absolute quantitation in various scientific fields.
  • Increasing sample throughput in HPLC necessitates advanced analytical methods for large-scale studies.
  • Current HPLC analysis methods struggle to keep pace with the growing volume of experimental data.

Purpose of the Study:

  • To develop a novel modular toolkit, HappyTools, for high-throughput targeted quantitation of HPLC measurements.
  • To automate critical steps in HPLC data analysis, including retention time calibration and data extraction.
  • To provide robust quality criteria for effective data curation in large HPLC studies.

Main Methods:

  • Development of the HappyTools software toolkit for automated HPLC data analysis.
  • Implementation of retention time calibration, data extraction, and quality criterion calculation within the workflow.
  • Testing and validation of HappyTools using a biopharmaceutical standard and previously published clinical samples.

Main Results:

  • HappyTools demonstrated comparable accuracy to established software like Waters Empower and ThermoFisher Chromeleon.
  • HappyTools significantly outperformed Waters Empower and ThermoFisher Chromeleon in terms of precision and throughput.
  • The toolkit successfully automated complex HPLC data analysis workflows, enhancing efficiency.

Conclusions:

  • HappyTools provides a powerful, automated solution for high-throughput targeted quantitation of HPLC data.
  • The toolkit offers improved precision and throughput, addressing the need for efficient analysis of large sample sets.
  • HappyTools is freely available, promoting wider adoption and advancement in HPLC-based quantitative analysis.