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Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

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Published on: January 26, 2013

Postmitotic Fate Refinement in the Subplate.

Mohammed A Mostajo-Radji¹, Alex A Pollen¹

¹Department of Neurology, University of California San Francisco, San Francisco, CA 94143, USA; The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA.

|July 7, 2018

Summary

Human pluripotent stem cells (hPSCs) reveal new insights into cortical neuron development. Projection neurons pause in the subplate, integrating cues for fate refinement before reaching deep cortical layers.

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Area of Science:

Neuroscience
Developmental Biology
Stem Cell Research

Background:

The astonishing diversity of cortical neurons arises from complex developmental processes.
Understanding neuronal fate specification is crucial for regenerative medicine and treating neurological disorders.

Purpose of the Study:

To investigate the developmental trajectory of human projection neurons.
To explore the role of the subplate in postmitotic neuronal fate refinement.

Main Methods:

Utilized human pluripotent stem cell (hPSC) neural differentiation.
Tracked the migration and behavior of projection neurons during cortical development.

Main Results:

Projection neurons exhibit prolonged periods in the subplate.
This subplate sojourn appears critical for integrating intrinsic and extrinsic signals.
Evidence suggests this pause facilitates fate refinement before migration to deep cortical layers.

Conclusions:

The subplate serves as a crucial "waiting" area for neuronal development.
Prolonged subplate residence allows for integration of developmental cues, refining neuronal identity.
This finding offers a novel perspective on human cortical neuron specification.