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Updated: Feb 8, 2026

Estimation of Plant Biomass Lignin Content using Thioglycolic Acid TGA
Published on: July 24, 2021
DcC4H and DcPER Are Important in Dynamic Changes of Lignin Content in Carrot Roots under Elevated Carbon Dioxide
Ya-Hui Wang1, Xue-Jun Wu1, Sheng Sun2
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , People's Republic of China.
Abstract:
In our study, isobaric tags for relative and absolute quantification (iTRAQ) was conducted to determine the significantly changed proteins in the fleshy roots of carrots under different carbon dioxide (CO2) treatments. A total of 1523 proteins were identified, of which 257 were differentially expressed proteins (DEPs). On the basis of annotation analysis, the DEPs were identified to be involved in energy metabolism, carbohydrate metabolism, and some other metabolic processes. DcC4H and DcPER, two lignin-related proteins, were identified from the DEPs. Under elevated CO2 stress, both carrot lignin content and the expression profiles of lignin biosynthesis genes changed significantly. The protein-protein interactions among lignin-related enzymes proved the importance of DcC4H and DcPER. The results of our study provided potential new insights into the molecular mechanism of lignin content changes in carrot roots under elevated CO2 stress.
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