Related Experiment Video
Updated: Feb 8, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
Abnormal activation of the sonic hedgehog signaling pathway in endometriosis and its diagnostic potency
Yanan He1, Qiuyan Guo1, Yan Cheng1
1Department of Gynecology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
Objective:
To investigate the abnormal expression of sonic hedgehog (SHH) signaling molecules in 52 eutopic endometrial tissues and its diagnostic potency in endometriosis.
Design:
Retrospective study.
Setting:
University hospital.
Patient(S):
Twenty-six women with histologically confirmed endometriosis and 26 women with histologically normal endometria who were undergoing curettage or hysterectomy were selected.
Intervention(S):
None.
Main Outcome Measure(S):
The mRNA and protein levels of molecules in the SHH signaling pathway.
Result(S):
The levels of SHH, smoothened, GLI family zinc finger 3, and its downstream signaling transcription factor (GLI1) not only were upregulated in the eutopic endometrium of endometriosis compared with the control endometrium, but also independently predicted the onset and severity of the disease.
Conclusion(S):
This study is the first to reveal differences in the activation of the SHH signaling pathway between women with and without endometriosis and suggests that the SHH signaling pathway has potential in the diagnosis of endometriosis.
Related Concept Videos
Hedgehog Signaling Pathway
Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Non-Canonical Wnt Signaling Pathways
Canonical Wnt Signaling Pathway
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...

