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Related Concept Videos

Cell Culture01:21

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction...
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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
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The total amount of energy acquired by primary producers in an ecosystem is called gross primary production (GPP). However, of this energy, producers use some for metabolic processes, and some is lost as heat, decreasing the amount of energy available to the next trophic level. The remaining usable amount of energy is called the net primary productivity (NPP). In terrestrial ecosystems, NPP is driven by climate, while light penetration and nutrient availability drive NPP in aquatic ecosystems.
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Adipogenesis in Primary Cell Culture.

Therese Juhlin Larsen1,2,3, Naja Zenius Jespersen1,2,3, Camilla Scheele4,5

  • 1The Centre of Inflammation and Metabolism and Centre for Physical Activity Research Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark.

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Summary

This study details isolating and culturing human brown and white preadipocytes in vitro. This method models adipogenesis, aiding research into obesity and novel therapeutic targets.

Keywords:
AdipogenesisBrown fatHuman brown adipocytesIn vitro differentiationObesityWhite fat

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Area of Science:

  • Adipose tissue biology
  • Obesity research
  • Cellular differentiation

Background:

  • Obesity is characterized by white adipose tissue expansion and brown adipose tissue loss.
  • Understanding adipocyte recruitment is crucial for developing anti-obesity strategies.
  • In vitro preadipocyte differentiation models in vivo adipogenesis.

Purpose of the Study:

  • To describe a step-by-step protocol for isolating and differentiating human brown and white preadipocytes in vitro.
  • To highlight considerations for this in vitro system.
  • To demonstrate its utility in modeling in vivo adipogenesis and identifying differential regulation.

Main Methods:

  • Isolation of primary brown and white preadipocytes from human tissue biopsies.
  • In vitro cell culture and differentiation protocols.
  • Analysis of adipogenesis using morphological and transcriptional events.

Main Results:

  • Successful isolation and differentiation of human brown and white preadipocytes in vitro.
  • Demonstration that depot-specific preadipocytes retain niche characteristics.
  • Establishment of an in vitro model for studying differential adipogenesis.

Conclusions:

  • The described in vitro system accurately models in vivo adipogenesis.
  • This model is valuable for investigating differential regulation between brown and white adipogenesis.
  • It offers a platform for discovering novel therapeutic targets for obesity.