Related Experiment Video
Updated: Feb 8, 2026

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
Published on: January 11, 2011
Selection of Molecular Markers for the Estimation of Somaclonal Variation
1Unidad de Genómica Avanzada (Langebio), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Guanajuato, México. octavio.martinez@cinvestav.mx.
Abstract:
Tissue culture for plant micropropagation is known to be a source of genetic changes termed "somaclonal variation". This protocol is designed to help in the selection of one or more types of molecular marker systems for the optimal detection and measurement of somaclonal variation. Somaclonal variation is influenced by the reproductive biology of the species, the number of individuals taken as tissue source, and the tissue culture protocol, while its detection and measurement depends upon the molecular marker system selected, which can also vary in the intensity of genome sampling. In turn, the intensity of genome sampling can be regulated varying parameters of the molecular technique. These factors are discussed and illustrated with in silico molecular marker protocols. Software, programed in R, to perform simulation and evaluation of somaclonal variation is made publicly available.
Related Concept Videos
What is Variation?
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
What is Natural Selection?
Variation
When independent and dependent variables are plotted on a scatter plot, the slope of a line is a value that describes the rate of change between the two...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Variation of Atmospheric Pressure
Assuming the air temperature is constant at a given altitude and that the ideal gas law of thermodynamics describes the atmosphere to a good approximation, one can find the variation of atmospheric pressure with height.
Let p(y) be the atmospheric pressure at...
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

