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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
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Electrochemically deposited silver detection substrate for surface-enhanced Raman spectroscopy cancer diagnostics
Zuzana Orságová Králová1, Andrej Oriňak1, Renáta Oriňaková1
1Pavol Jozef Šafárik Univ. in Košice, Slovakia.
Journal of Biomedical Optics
|July 8, 2018
Summary
This study presents a new, label-free diagnostic test for colorectal cancer using surface-enhanced Raman spectroscopy (SERS) on a silver surface. The method shows high accuracy, offering a rapid and inexpensive primary screening tool.
Area of Science:
- Analytical Chemistry
- Biomedical Diagnostics
- Materials Science
Background:
- Colorectal cancer (CRC) diagnosis often relies on invasive procedures.
- Label-free, noninvasive diagnostic methods are highly desirable for early detection.
- Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity for molecular detection.
Purpose of the Study:
- To develop and evaluate a novel SERS-based diagnostic test for colorectal cancer using blood plasma.
- To assess the feasibility of using electrochemically prepared silver surfaces for SERS analysis of blood plasma.
- To determine the diagnostic accuracy (sensitivity and specificity) of the developed SERS method for CRC detection.
Main Methods:
- Indium tin oxide glass substrates were modified with silver nitrate using a pulsed double-potentiostatic method to create silver dendritic surfaces.
- The optimal silver substrate was selected based on signal enhancement using Rhodamine 6G as a model analyte.
- SERS spectra of blood plasma from colorectal cancer patients (n=15) and healthy volunteers (n=15) were acquired and statistically analyzed.
Main Results:
- Biomolecular changes in blood plasma indicative of colorectal cancer were detected via SERS spectral analysis.
- A diagnostic accuracy of 94% specificity and 100% sensitivity was achieved using the ratio of SERS peak intensities (adenine to tyrosine).
- Principal component analysis further demonstrated 100% specificity and sensitivity for differentiating cancer patients from controls.
Conclusions:
- The developed SERS method using electrochemically prepared silver surfaces is a rapid, inexpensive, and effective label-free diagnostic tool for colorectal cancer.
- This approach eliminates the need for nanoparticle preparation and plasma incubation, simplifying the diagnostic workflow.
- The SERS-based method shows significant potential for introduction as a primary screening test for colorectal cancer.
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