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Updated: Feb 8, 2026
Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
Techniques for chromosomal integration and expression optimization in Escherichia coli
Bingming Ou1,2,3,4, Carolina Garcia4, Yejun Wang5
1College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
Abstract:
Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.
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