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Published on: November 26, 2017
Crosslinked enzyme aggregates (CLEA) of phytase with soymilk proteins
Harini Tirunagari1, Shalini Basetty2, Haridas B Rode1
1Organic Synthesis and Process Chemistry Division, Indian Institute of Chemical Technology, Hyderabad, 500 007, India; Academy of Scientific and Innovative Research (AcSIR), CSIR - Indian Institute of Chemical Technology, Hyderabad, India.
Abstract:
Crosslinked Enzyme Aggregates (CLEA) of phytase (E.C. 3.1.3.8) have been successfully prepared using soymilk as protein feeder. Compared to total initial activity of 4428 units used for immobilization, the recovered activity in the CLEA is enhanced to 8000 units. The CLEA retained 100% phytase activity up to 50 °C and 70% activity at 70 °C after 6 h incubation while soluble enzyme lost 50% activity at 50 °C and 80% at 70 °C. Soluble phytase has an optimum pH of 2.1 while immobilized enzyme maintains high level of activity in a broad pH range of 1-6. CLEA also retain 80% activity upon proteolytic hydrolysis with pepsin, pancreatin, trypsin and chymotrypsin, while soluble enzyme loses 40% activity under same conditions. Phytase CLEA were recycled for 5 times without loss in activity using a stirred basket reactor.
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