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Updated: Feb 8, 2026

Quantitation of Intra-peritoneal Ovarian Cancer Metastasis
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Quantitation of Intra-peritoneal Ovarian Cancer Metastasis

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Cancer-related transcription regulator protein NAC1 forms a protein complex with CARM1 for ovarian cancer progression

Naomi Nakayama1, Gyosuke Sakashita1, Yuko Nariai1

  • 1Department of Biochemistry, Shimane University School of Medicine, Izumo, Japan.

Oncotarget
|July 10, 2018
PubMed

Insights

Nuclear Factor NAC1 (NAC1) and CARM1 protein complex formation in ovarian cancer cells is linked to poor prognosis. High NAC1 and CARM1 expression predicts chemotherapy resistance, identifying them as potential prognostic biomarkers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Nuclear Factor NAC1 (NAC1) is a transcription regulator overexpressed in multiple carcinomas.
  • NAC1 overexpression correlates with poor outcomes and chemotherapy resistance in ovarian cancer.
  • The protein complex formation of intranuclear NAC1 in ovarian cancer cells is not well understood.

Purpose of the Study:

  • To investigate the protein complex formation of NAC1 in ovarian cancer cells.
  • To identify NAC1 interacting partners.
  • To evaluate the prognostic significance of NAC1 and its interacting partners in ovarian cancer.

Main Methods:

  • Ovarian cancer cell lysates were analyzed using Fast Protein Liquid Chromatography (FPLC).
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify interacting proteins.
  • Tissue microarray analysis was performed to correlate protein expression with patient prognosis.

Main Results:

  • NAC1 was found to exist in a larger protein complex (300-500 kDa) than its individual molecular mass (58 kDa).
  • CARM1 was identified as a novel binding partner of NAC1 within this complex.
  • High expression levels of both NAC1 and CARM1 significantly correlated with poor prognosis in ovarian cancer patients receiving chemotherapy.

Conclusions:

  • NAC1 forms a larger protein complex with CARM1 in ovarian cancer cells.
  • The combined high expression of NAC1 and CARM1 serves as a prognostic biomarker for chemotherapy resistance in ovarian cancer.
  • Targeting NAC1 and CARM1 may offer therapeutic strategies for overcoming chemotherapy resistance.
Keywords:
coactivator-associated arginine methyltransferase 1 (CARM1)nucleus accumbens-associated protein 1 (NAC1)ovarian cancerprotein arginine N-methyltransferase 4 (PRMT4)

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