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Updated: Feb 8, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
Published on: December 29, 2017
Real-time visualization of two-photon fluorescence lifetime imaging microscopy using a wavelength-tunable femtosecond
Jiheun Ryu1,2, Ungyo Kang3, Jayul Kim1
1Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
Abstract:
A fluorescence lifetime imaging microscopy (FLIM) integrated with two-photon excitation technique was developed. A wavelength-tunable femtosecond pulsed laser with nominal pulse repetition rate of 76-MHz was used to acquire FLIM images with a high pixel rate of 3.91 MHz by processing the pulsed two-photon fluorescence signal. Analog mean-delay (AMD) method was adopted to accelerate the lifetime measurement process and to visualize lifetime map in real-time. As a result, rapid tomographic visualization of both structural and chemical properties of the tissues was possible with longer depth penetration and lower photo-damage compared to the conventional single-photon FLIM techniques.
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