The ved protein patterning in zebrafish embryos

Homeobox transcription factors play an essential role in cells differentiation. The function is realized by the proteins (not by the mRNA) and it is necessary to pay more attention to the protein patterns. In this study we were the first to obtain antibodies against the ved protein, tested their specificity by Western-blot analysis and performed a whole mount immunostaining of zebrafish embryos. It was shown that the spatial-temporal ved protein pattern did not differ from that of other vent-family factors. And moreover, its synthesis like that of vox and vent did not depend on pou5f3.