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MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1

Quanxiang Liu1, Yang Song1, Xianliang Duan1

  • 1Department of Orthopedics, The Affiliated Hospital of Beihua University, Jilin, China.

Biomed Research International
|July 10, 2018
PubMed
Abstract

Insights

MicroRNA-92a (miR-92a) inhibits osteosarcoma (OS) growth and migration by targeting Notch1. This finding offers a potential new strategy for OS diagnosis and treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRs) play a role in osteosarcoma (OS) development and progression.
  • Investigating specific miRs like miR-92a is crucial for understanding OS pathogenesis.
  • This study focuses on miR-92a's regulatory function in OS.

Purpose of the Study:

  • To elucidate the role of miR-92a in osteosarcoma (OS) development.
  • To explore miR-92a as a potential diagnostic and therapeutic target for OS.
  • To identify the molecular mechanisms underlying miR-92a's function in OS.

Main Methods:

  • Cell viability assessed using MTT assay.
  • Cell cycle and apoptosis analyzed by flow cytometry.
  • Protein expression evaluated via Western blot; RNA expression by qPCR; cell migration using Transwell assay.

Main Results:

  • MiR-92a inhibited osteosarcoma (OS) cell proliferation and migration in vitro.
  • MiR-92a reduced tumor volume in vivo and enhanced cisplatin sensitivity in OS.
  • MiR-92a was identified as a direct target of Notch1.

Conclusions:

  • MiR-92a inhibits OS cell growth and migration through Notch1.
  • MiR-92a enhances cisplatin sensitivity in osteosarcoma (OS) cells.
  • Targeting miR-92a presents a novel therapeutic strategy for osteosarcoma (OS).

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