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Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs
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The Nuclear RNA-binding Protein RBSR1 Interactome in Trypanosoma cruzi

Helisa H Wippel1, Juliane S Malgarin2, Sharon de Toledo Martins1

  • 1Carlos Chagas Institute, FIOCRUZ, Rua Professor Algacyr Munhoz Mader 3775, Curitiba, Paraná, Brazil.

The Journal of Eukaryotic Microbiology
|July 10, 2018
PubMed

Insights

The study identifies RBSR1, a novel RNA-binding protein in Trypanosoma cruzi, crucial for regulating gene expression. RBSR1

Area of Science:

  • Molecular Biology
  • Parasitology
  • Eukaryotic Gene Regulation

Background:

  • Trypanosoma cruzi causes Chagas disease, making it a significant medical and research model.
  • Post-transcriptional regulation via messenger ribonucleoprotein (mRNP) complexes is vital in eukaryotes.
  • RBSR1, a serine-arginine (SR)-rich RNA-binding protein (RBP), is identified in T. cruzi.

Purpose of the Study:

  • To investigate the function and interactions of the RBSR1 protein in T. cruzi.
  • To explore the potential role of RBSR1 in RNA processing and gene regulation.

Main Methods:

  • Phylogenetic analysis to identify orthologous proteins.
  • Protein interaction studies with other RBPs.
  • Ribonomics (immunoprecipitation followed by sequencing) to identify bound RNAs.

Main Results:

  • RBSR1 is a nuclear, developmentally regulated RBP in T. cruzi.
  • RBSR1 interacts with known RBPs like UBP1, UBP2, and TRRM1.
  • Ribonomics data revealed enrichment of small nucleolar RNAs (snoRNAs) and small nuclear RNAs (snRNAs) in the RBSR1 complex.

Conclusions:

  • RBSR1 is phylogenetically related to human splicing factor SRSF7.
  • Evidence suggests RBSR1's involvement in nuclear RNA processing pathways in T. cruzi.
  • RBSR1 plays a role in post-transcriptional regulation within T. cruzi.
Keywords:
Gene expression regulationimmunoprecipitation complexnucleus

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