Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

6.6K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
6.6K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

32.8K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.8K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.2K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.2K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.8K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.8K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

27.1K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.1K
Transcription01:10

Transcription

157.0K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
157.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thyroid hormones maintain parvalbumin neuron functions in the mouse neocortex.

iScience·2026
Same author

Beyond seizure control: Identifying deficits in cognitive networks in absence epilepsy.

Science advances·2026
Same author

Disrupted Development of the mPFC-Thalamic Circuit in Shank3<sup>-/-</sup> mice, an autism-associated model.

Molecular psychiatry·2025
Same author

Elevated pyramidal cell firing orchestrates arteriolar vasoconstriction through COX-2-derived prostaglandin E2 signaling.

eLife·2025
Same author

The reuniens thalamus recruits recurrent excitation in the medial prefrontal cortex.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Astrocyte aquaporin mediates a tonic water efflux maintaining brain homeostasis.

eLife·2024

Related Experiment Video

Updated: Feb 8, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

10.4K

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp.

Gabrielle Devienne1, Benjamin Le Gac1, Juliette Piquet1

  • 1UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), Sorbonne Université.

Journal of Visualized Experiments : Jove
|July 10, 2018
PubMed
Summary

This study presents a multiplex single cell reverse transcription polymerase chain reaction (RT-PCR) protocol. This method enables simultaneous gene expression analysis in individual cerebral cortex cells after patch-clamp recording, aiding cell type characterization.

More Related Videos

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
08:10

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Published on: May 2, 2019

8.9K
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.6K

Related Experiment Videos

Last Updated: Feb 8, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

10.4K
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
08:10

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Published on: May 2, 2019

8.9K
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.6K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • The cerebral cortex contains diverse cell types with varied features, complicating their identification and functional study.
  • Characterizing individual cell types is crucial for understanding brain function.

Purpose of the Study:

  • To describe a multiplex single cell reverse transcription polymerase chain reaction (RT-PCR) protocol.
  • To enable simultaneous detection of tens of genes in single cells for improved cell type characterization.

Main Methods:

  • Patch-clamp recording in brain slices followed by cytoplasmic content harvesting.
  • Single-cell reverse transcription and multiplex polymerase chain reaction (PCR) for gene expression analysis.
  • Integration with morphological characterization for comprehensive phenotypic analysis.

Main Results:

  • The protocol allows simultaneous detection of multiple gene expressions in single cells.
  • It can be combined with morphological and electrophysiological data.
  • Applicable to studying cell types in specific microenvironments, e.g., near blood vessels.

Conclusions:

  • Multiplex single cell RT-PCR is a valuable tool for identifying and characterizing diverse neuronal cell types.
  • The method is widely applicable and accessible to electrophysiology laboratories with careful implementation.
  • Facilitates detailed study of cellular heterogeneity and function in the brain.