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[Adaptation to the cell line PLC/PRF/5 of hepatitis A virus released in the cell culture medium]
Abstract:
The propagation of hepatitis A virus (HAV), CF53 strain, released without any cytopathic effect into the PLC/PRF/5 cells supernatant, was studied in the course of six serial passages (6th to 11th). The decrease (from 5 to 1 week) of incubation time required to detect HAV, by RIA, in culture supernatant, the increase in Hepatitis A antigen (from 777 to 10,038 c.p.m./50 microliter) and infectivity titre (from 10(3.0) TCID 50/ml to 10(4.5) TCID 50/ml) were consistent with the adaptation of this virus to the cell line PLC/PRF/5.
Insights
Hepatitis A virus (HAV) CF53 strain adapted to PLC/PRF/5 cells over six passages. This adaptation showed reduced incubation times, increased antigen levels, and higher infectivity titers, indicating successful viral propagation.
Area of Science:
- Virology
- Cell Biology
- Hepatitis A Virus Research
Context:
- Investigating the propagation of Hepatitis A virus (HAV), specifically the CF53 strain.
- Studying viral behavior in the PLC/PRF/5 cell line without observable cytopathic effects.
Purpose:
- To determine the adaptation of HAV CF53 strain to the PLC/PRF/5 cell line through serial passages.
- To quantify changes in viral detection, antigen levels, and infectivity during adaptation.
Summary:
- Serial passaging (6th to 11th) of HAV CF53 in PLC/PRF/5 cells led to viral adaptation.
- Key indicators of adaptation included a decrease in incubation time for detection (5 to 1 week) via RIA.
- Significant increases in Hepatitis A antigen levels (777 to 10,038 c.p.m./50 microliter) and infectivity titer (10(3.0) to 10(4.5) TCID 50/ml) were observed.
Impact:
- Demonstrates successful adaptation of HAV to a specific cell line, facilitating further virological studies.
- Provides quantitative data on viral propagation and infectivity changes during cell line adaptation.
- Highlights the utility of PLC/PRF/5 cells for studying HAV replication and characteristics.