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Updated: Feb 8, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
Published on: February 23, 2021
Synergy of MS2 disinfection by sequential exposure to tailored UV wavelengths
Natalie M Hull1, Karl G Linden1
1Department of Civil, Environmental, and Architectural Engineering, University of Colorado, Boulder, 4001 Discovery Dr, UCB 607, Boulder, CO, 80303, USA.
Abstract:
The advantages of polychromatic ultraviolet (UV) light for viral disinfection can be optimized for disinfection using emerging tailored wavelength sources including KrCl excimer lamps and light emitting diodes (LEDs). Disinfection of the common viral surrogate MS2 bacteriophage was measured after exposure to these emerging sources and conventional low pressure (LP) mercury UV lamps in individual or sequential exposures. The first dose response for any virus (MS2) exposed to a KrCl excimer lamp is reported, showing the high efficiency of fluence-based disinfection because of increased viral susceptibility at the low wavelengths emitted by the excilamp. Sequential exposure dose responses indicated synergy from sequential exposures of LP and excimer lamps, which were competitive on an electrical basis at worst-and best-case scenarios of wall plug efficiency with current medium pressure (MP) disinfection. Best-case scenarios for electrical efficiency also showed all sequential exposures to be competitive with MP UV disinfection. Predictive models for sequential exposure dose responses were assessed to support the current feasibility of incorporating sequential UV exposures to optimize tailored wavelength viral disinfection.
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