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Updated: Feb 8, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Published on: April 14, 2015
Development of a TaqMan probe-based quantitative reverse transcription PCR assay for detection of Getah virus RNA
Ning Shi1, Hao Liu2, Li-Xia Li3
1Key Laboratory of Special Animal Epidemic Disease, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Changchun, Jilin, China. shiningtcs@126.com.
Abstract:
Getah virus (GETV), a mosquito-borne virus that mainly infects horses and pigs, has emerged and spread in China. We developed a highly specific and reproducible TaqMan probe-based quantitative reverse transcription PCR (RT-qPCR) assay targeting the non-structural protein 1 of GETV, whose detection limit is 25.5 copies/µL, which is 100-fold higher than that of conventional RT-PCR. RT-qPCR was used to detect GETV RNA in mosquito and animal clinical samples, showing that the accuracy of RT-qPCR was higher than that of conventional RT-PCR. The newly developed RT-qPCR assay may be a useful alternative tool for rapid, simple and specific diagnosis of GETV infection.
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