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Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy

Serena Flori1,2, Pierre-Henri Jouneau3, Benoit Gallet4

  • 1Laboratoire de Physiologie Cellulaire et Végétale, Centre National de la Recherche Scientifique, Institut National Recherche Agronomique, Commissariat à l'Energie Atomique et aux Energies Alternatives, CEA Grenoble, UMR5168, Université Grenoble Alpes, Grenoble, France.

Methods in Molecular Biology (Clifton, N.J.)
|July 11, 2018
PubMed
Summary

Advanced microscopy techniques offer new ways to study internal chloroplast structures. This study details methods for visualizing chloroplast morphology in 3D using focused ion beam-scanning electron microscopy (FIB-SEM).

Keywords:
ArabidopsisConfocal imagingFocused ion beam (FIB)Phaeodactylum tricornutumScanning electron microscopy (SEM)TomographyTransmission electron microscopy (TEM)

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