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Transgenic Organisms

Transgenic Organisms

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Conditions on Early Earth

Conditions on Early Earth

Around 4 billion years ago, oceans began to condense on earth while volcanic eruptions released nitrogen, carbon dioxide, methane, ammonia, and hydrogen into the primordial atmosphere. However, organisms with the characteristics of life were not initially present on earth. Scientists have used experimentation to determine how organisms evolved that could grow, reproduce, and maintain an internal environment.

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Related Experiment Video

Updated: Feb 8, 2026

Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Published on: November 26, 2014

Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches.

Alexandra L McCubbrey^1,2, William J Janssen^3,4

¹Department of Medicine, National Jewish Health, Denver, CO, USA. McCubbreyA@njhealth.org.

Methods in Molecular Biology (Clifton, N.J.)

|July 11, 2018

Summary

Transgenic mouse models, utilizing Cre/Lox technology, offer precise control over lung myeloid cell research. This overview details conditional and inducible systems for studying lung immunity and disease.

Keywords:

Breeding Conditional Cre CreER Inducible ROSA26 Transgenic mice rtTA

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Last Updated: Feb 8, 2026

Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

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Published on: January 29, 2019

Generation of Transgenic Rats using a Lentiviral Vector Approach

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Area of Science:

Immunology
Genetics
Animal Models

Background:

Transgenic mice are crucial for investigating lung myeloid cell functions in health and disease.
Cre/Lox site-specific recombination enables targeted gene deletion in specific locations.
Inducible triggers refine Cre/Lox systems for spatial and temporal control.

Purpose of the Study:

To review available conditional and inducible transgenic mouse strains targeting lung myeloid cells.
To guide researchers on breeding schemes and controls for manipulating myeloid cell function.

Main Methods:

Overview of commercially available transgenic mouse lines.
Description of Cre/Lox and inducible Cre/Lox systems.
Guidance on breeding strategies and experimental controls.

Main Results:

Identification of various transgenic mouse models for lung myeloid cell research.
Explanation of how these models allow for precise genetic manipulation.
Provision of practical advice for experimental design.

Conclusions:

Conditional and inducible transgenic mice are powerful tools for studying lung myeloid cells.
Appropriate use of these models and breeding schemes is essential for successful research.
This resource aids researchers in modulating myeloid cell function for disease and homeostasis studies.