Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.5K
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

1.3K
Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
1.3K
Leaky Scanning02:28

Leaky Scanning

5.7K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.7K
IR Frequency Region: X–H Stretching01:24

IR Frequency Region: X–H Stretching

1.5K
In IR spectroscopy, signals produced by the X−H bonds (such as C−H, O−H, or N−H) can be observed in the frequency range of  2700–4000 cm–1. The C−H stretching vibration forms sharp bands in the region 2850–3000 cm–1. The presence of the O−H stretching vibration leads to the forming of an absorption band in the frequency range 3650–3200 cm−1. At the same time, N−H stretching can be confirmed by absorption bands in...
1.5K
IR Frequency Region: Alkyne and Nitrile Stretching01:22

IR Frequency Region: Alkyne and Nitrile Stretching

1.5K
Both alkyne (C≡C) and nitrile (C≡N) functional groups contain triple bonds and show stretching absorptions around the wavenumber range of 2100 to 2300 cm−1 in the diagnostic region of the IR spectra.
Comparing the stretching vibrational frequency of  C≡C triple bonds with that of double and single bonds, it is evident that C≡C triple bonds exhibit a higher stretching frequency than C=C double and C–C single bonds. Similarly, the C≡N triple bond...
1.5K
IR Frequency Region: Alkene and Carbonyl Stretching01:29

IR Frequency Region: Alkene and Carbonyl Stretching

1.3K
Double bonds in alkenes and carbonyl compounds exhibit stretching frequencies in the diagnostic region of the IR spectrum. In addition, alkenes exhibit vinylic C–H stretching and C–H out-of-plane bending absorptions that are useful for identifying substitution patterns.
Stretching frequencies are affected by several factors, such as resonance, inductive effects, ring strain, dipole moment, and hydrogen bonding. Consequently, the stretching frequency of the carbonyl double bond...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An autonomous intelligent mosquito sentinel for field-deployed surveillance.

Communications engineering·2026
Same author

Multi-View Pareto Optimization for Minimal-Diagnostic-Set Identification of Disease Vectors.

Insects·2026
Same author

Seeing through arthropod eyes: An AI-assisted, biomimetic approach for high-resolution, multi-task imaging.

Science advances·2025
Same author

Concentration Measurement with Ultrabroad Dynamic Range Using Few-Step Variable Optical-Path-Length Slope Method.

Analytical chemistry·2025
Same author

Deep learning-enabled filter-free fluorescence microscope.

Science advances·2025
Same author

A Miniature Modular Fluorescence Flow Cytometry System.

Biosensors·2024

Related Experiment Video

Updated: Feb 8, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.7K

Ultrafast cell edge detection by line-scan time-stretch microscopy.

Bo Dai1, Lu He1, Lulu Zheng1

  • 1Engineering Research Center of Optical Instrument and System, the Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai, China.

Journal of Biophotonics
|July 11, 2018
PubMed
Summary

A new ultrafast time-stretch imaging method enhances cell classification by detecting edges using differential detection. This high-throughput technique reduces computational complexity for rapid analysis of various cell types.

Keywords:
cell analysisedge detectionpattern recognitionultrafast imaging

More Related Videos

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
10:25

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

Published on: December 9, 2011

18.2K
Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy
08:41

Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy

Published on: June 13, 2025

1.2K

Related Experiment Videos

Last Updated: Feb 8, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.7K
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
10:25

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

Published on: December 9, 2011

18.2K
Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy
08:41

Equibiaxial Stretching Device for High Magnification Live-Cell Confocal Fluorescence Microscopy

Published on: June 13, 2025

1.2K

Area of Science:

  • Biomedical Optics
  • Image Processing
  • Cell Biology

Background:

  • Ultrafast time-stretch imaging offers high throughput and sensitivity for cell classification.
  • Existing methods may have high computational complexity for edge extraction.

Purpose of the Study:

  • To propose a novel time-stretch imaging modality for efficient edge detection in cells.
  • To reduce computational complexity in image processing for cell analysis.

Main Methods:

  • Utilized differential detection for edge detection based on directional derivatives.
  • Implemented image processing primarily in the physical layer.
  • Developed an imaging system with a scan rate of 77.76 MHz.

Main Results:

  • Successfully demonstrated edge extraction feasibility using a resolution target.
  • Detected various cell types, including red blood cells, lung cancer cells, and breast cancer cells.
  • Observed distinct edge patterns for cancerous cells.

Conclusions:

  • The proposed imaging system enables efficient, low-complexity edge detection.
  • This technique is a promising candidate for high-throughput cell classification.
  • Distinct edge features of cancerous cells can aid in their identification.