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Updated: Feb 8, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
Genome-wide SWAp-Tag yeast libraries for proteome exploration
Uri Weill1, Ido Yofe1, Ehud Sass2
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
None:
Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.
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