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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
OsMATL mutation induces haploid seed formation in indica rice
Li Yao1, Ya Zhang1, Chunxia Liu1
1Syngenta Beijing Innovation Center, ZhongGuanCun Life Science Park, Beijing, China.
Abstract:
Intraspecific haploid induction in maize (Zea mays) is triggered by a native frameshift mutation in MATRILINEAL (MATL), which encodes a pollen-specific phospholipase. To develop a haploid inducer in rice (Oryza sativa), we generated an allelic series in the putative ZmMATL orthologue, OsMATL, and found that knockout mutations led to a reduced seed set and a 2-6% haploid induction rate. This demonstrates MATL functional conservation and represents a major advance for rice breeding.
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