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Plasticity-first evolution explains how new traits emerge by refining existing adaptability. This study shows how diet-induced plasticity in amphibians led to a novel carnivore form, supporting this evolutionary theory.

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Area of Science:

  • Evolutionary Biology
  • Developmental Biology
  • Ecology

Background:

  • Plasticity-first evolution (PFE) proposes that novel traits originate from selection acting on pre-existing phenotypic plasticity.
  • Empirical evidence for PFE in natural populations remains limited, making its role in evolutionary innovation debated.

Purpose of the Study:

  • To investigate the role of PFE in the evolution of a novel carnivore morph in spadefoot toad tadpoles.
  • To compare the developmental plasticity and adaptive refinement of this trait across three species with varying carnivore production.

Main Methods:

  • Comparative analysis of tadpole morphology, gene expression, and growth across three spadefoot toad species (Scaphiopus holbrookii, Spea multiplicata, Spea bombifrons).
  • Tadpoles were reared on alternative diets to assess diet-induced phenotypic plasticity and its subsequent refinement.
  • Examined gene expression patterns related to diet-induced morphological changes.

Main Results:

  • Scaphiopus holbrookii exhibited diet-induced plasticity in morphology and gene expression, consistent with initial PFE.
  • Spea multiplicata showed adaptive refinement of diet-induced plasticity, progressing towards the carnivore morph.
  • Spea bombifrons displayed further refinement of the carnivore phenotype, often independent of diet, indicating advanced PFE.

Conclusions:

  • The study provides strong evidence for PFE driving the evolution of a novel carnivore morph in amphibians.
  • Phenotypic plasticity plays a crucial, though often underestimated, role in fostering evolutionary innovation and niche expansion.
  • The findings support the broader significance of PFE in generating evolutionary novelties across diverse taxa.