Related Experiment Video
Updated: Feb 8, 2026

Evidence-based Knowledge Synthesis and Hypothesis Validation: Navigating Biomedical Knowledge Bases via Explainable AI and Agentic Systems
Published on: June 13, 2025
Validity and Reliability of the Glaucoma Knowledge Level Questionnaire
Zeynep Demirtaş1, Gökçe Dağtekin1, Muhammed Fatih Önsüz1
1Eskişehir Osmangazi University Faculty of Medicine, Department of Public Health, Eskişehir, Turkey.
Objectives:
The present study was conducted to develop an instrument for measuring adults' glaucoma knowledge levels and to establish the instrument's validity and reliability.
Materials And Methods:
The study group consisted of 811 persons aged 40-80 years who presented to primary health care institutions and did not have a glaucoma diagnosis. A 27-item questionnaire measuring level of glaucoma knowledge was created by the study team. Following expert consultation, it was structurally evaluated. The difficulty index and discrimination index were calculated for each item. Factor analysis was used to determine construct validity, Cronbach's alpha internal consistency coefficient and item-total correlations were calculated to determine reliability. Confirmatory factor analysis was used to assess the extent to which the factor structure of the scale fit. We analysed correlation with the National Eye Health Education Program (NEHEP) Eye-Q scale in order to evaluate the validity of the scale.
Results:
The final glaucoma knowledge level questionnaire comprised 10 items in one dimension. The discrimination index and difficulty index ranged between 0.28 to 0.65 and 33 to 61%, respectively. According to factor analysis, the Kaiser-Meyer-Olkin score was 0.760 and Bartlett's test indicated p<0.001. Confirmatory factor analysis showed acceptable scale fit and fit indices. Validity assessment revealed a positive correlation between the total score of the items of the NEHEP scale and glaucoma knowledge level questionnaire score (r=0.522; p<0.001). Scores were higher in participants who were aged 40-64, living in the city, had education level of high school or above and had previous eye examination or intraocular pressure measurement.
Conclusion:
The glaucoma knowledge level questionnaire has the distinction of being the first valid and reliable scale for assessing level of glaucoma knowledge in Turkey.
Related Concept Videos
Reliability and Validity
Glaucoma: Overview
Open Angle Glaucoma: Treatment
Drugs such as carbonic anhydrase inhibitors, α2- and...
Distribution Reliability and Automation
Angle Closure Glaucoma: Treatment
Data Validation
Key parameters for method validation include:

