GLC determination of indoprofen in plasma

Area of Science:

• Pharmacokinetics
• Analytical Chemistry
• Drug Metabolism

Background:

• Human bioavailability studies for the analgesic indoprofen necessitate a precise and efficient analytical method.
• Existing methods may lack the required sensitivity or convenience for plasma drug level monitoring.

Purpose of the Study:

• To develop and validate a rapid, sensitive, and convenient assay for quantifying indoprofen in human blood plasma.
• To support human bioavailability studies by providing reliable plasma concentration data.

Main Methods:

• Ether extraction of acidified plasma samples.
• Derivatization of extracted indoprofen using diazomethane.
• Gas-liquid chromatography (GLC) analysis utilizing a 3% OV-1 column.

Main Results:

• The developed assay demonstrated sufficient sensitivity for measuring indoprofen concentrations in the range of 0.4-16 µg/ml.
• An average recovery rate of 99.0% ± 7.6% was achieved.
• The pentanoic acid homolog proved effective as an internal standard.

Conclusions:

• The described ether extraction, diazomethane derivatization, and GLC method is suitable for the determination of indoprofen in human plasma.
• This assay provides a reliable tool for pharmacokinetic and bioavailability assessments of indoprofen.