Mouse polyoma virus and adenovirus replication in mouse cells temperature-sensitive in DNA synthesis

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Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
11:13

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

Published on: March 28, 2012

R Sheinin, J Fabbro, M Dubsky

    Intervirology
    |January 1, 1985
    PubMed

    Insights

    Mouse adenovirus replicates in mouse cells lacking key DNA replication factors. This suggests viral DNA replication is independent of DNA topoisomerase II and G1/S interface traverse proteins.

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Mouse adenovirus is a common viral agent in laboratory mice.
    • Cell cycle progression is crucial for viral DNA replication in many viruses.
    • Specific genetic loci (tsA1S9, tsC1, ts2) encode essential host factors for DNA replication and cell cycle progression.

    Purpose of the Study:

    • To investigate the replication mechanism of mouse adenovirus in host cells with impaired DNA replication machinery.
    • To determine if mouse adenovirus DNA replication is dependent on host DNA topoisomerase II and G1/S interface proteins.

    Main Methods:

    • Utilizing temperature-sensitive mutant mouse fibroblast cell lines (tsA1S9, tsC1, ts2) with defects in DNA replication and cell cycle.
    • Infecting these mutant cells with mouse adenovirus at non-permissive temperatures.
    • Observing and quantifying viral multiplication and DNA replication.

    Main Results:

    • Mouse adenovirus successfully replicated in tsA1S9, tsC1, and ts2 mouse fibroblasts, even at temperatures where host cell DNA replication was inhibited.
    • Viral DNA replication proceeded without impediment in the absence of functional DNA topoisomerase II, a DNA-chain-elongation factor, and a protein essential for G1/S interface traverse.

    Conclusions:

    • Mouse adenovirus DNA replication is independent of critical host cell DNA replication and cell cycle control factors.
    • These findings offer insights into the unique replication strategies of adenoviruses and can be compared to other viruses like polyoma virus.

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