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Updated: Feb 8, 2026

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
Published on: November 28, 2019
High selective and sensitive detection of Zn(II) using tetrapeptide-based dansyl fluorescent chemosensor and its
Peng Wang1, Dagang Zhou1, Bo Chen1
1Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong 637002, PR China.
Abstract:
The zinc ions (Zn2+) play extremely irreplaceable role in the organism and the environment, the design and synthesis of a biomolecule-based fluorescence chemosensor for the detection of Zn2+ with high sensitivity is very important. Herein, a novel tetrapeptide-based dansyl fluorescent "turn-on" response chemosensor (L) has been designed and synthesized by solid phase peptide synthesis (SPPS). As designed, L can detect Zn2+ ions with specifically and sensitively based on photo-induced electron transfer (PET) mechanism in 100% aqueous solutions, and other metal ions do not interfere with Zn2+ ions recognition. The stoichiometric ratio of L with Zn2+ ions was 2:1, which matches with fluorescence titration and Job-plot assay. In addition, the reversibility and circularly process of the detection of L was confirmed by adding bonding agent Na2EDTA. Moreover, L exhibits excellent biocompatibility and low biotoxicity with the limit of detection (LOD) for Zn2+ about 18 nM, and has been successfully utilized for fluorescence imaging of Zn2+ ions in living HeLa cells under physiological conditions.
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