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The mesenchymoangioblast, mesodermal precursor for mesenchymal and endothelial cells
Igor I Slukvin1,2,3, Akhilesh Kumar4
1Wisconsin National Primate Research Center, University of Wisconsin, 1220 Capitol Ct., Madison, WI, 53715, USA. islukvin@wisc.edu.
Cellular and Molecular Life Sciences : CMLS
|July 12, 2018
Summary
Mesenchymoangioblasts (MBs) are the earliest endothelial and mesenchymal cell precursors from human pluripotent stem cells. These cells form distinct colonies and can differentiate into various mesenchymal cell types, valuable for regenerative medicine.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Mesenchymoangioblast (MB) identified as the earliest precursor for endothelial and mesenchymal cells.
- MBs originate from APLNR+PDGFRα+KDR+ mesoderm in human pluripotent stem cell (hPSC) cultures.
- MBs are characterized by their ability to form FGF2-dependent spheroid colonies.
Purpose of the Study:
- To review the specification and developmental potential of MBs.
- To differentiate MBs from other described mesenchymal progenitors.
- To discuss the implications for identifying molecular pathways and developing cellular therapies.
Main Methods:
- Clonogenic cultures of hPSCs in serum-free semisolid medium.
- Characterization of MB colonies using cell surface markers (e.g., PDGFRβ, CD271, EMCN, DLK1, CD73).
- Assessment of differentiation potential into mesenchymal stromal/stem cells (MSCs), pericytes, and smooth muscle cells.
Main Results:
- MB colonies comprise PDGFRβ+CD271+EMCN+DLK1+CD73- primitive mesenchymal cells.
- These cells arise from endothelial/angioblastic intermediates within 3-4 days.
- MB-derived cells show potential for differentiation into MSCs, pericytes, and smooth muscle cells.
Conclusions:
- MBs represent a distinct early progenitor population with significant developmental potential.
- Understanding MB specification aids in elucidating MSC and vasculogenic cell development.
- MBs offer promise for developing novel cellular therapies.
Keywords:
Cardiovascular progenitorsEmbryonic mesenchymeEmbryonic stem cellsHemangioblastsHuman pluripotent stem cellsInduced pluripotent stem cellsMesenchymal stem cellsMesenchymoangioblastsMesoangioblastMesoderm developmentMesospheresPericytesSmooth musclesMore Related Videos
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