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A Fully Automated Continuous-Flow Platform for Fluorescence Quenching Studies and Stern-Volmer Analysis
Koen P L Kuijpers1, Cecilia Bottecchia1, Dario Cambié1
1Department of Chemical Engineering and Chemistry, Sustainable Process Engineering, Micro Flow Chemistry & Process Technology, Eindhoven University of Technology, Den Dolech 2, 5612, AZ, Eindhoven, The Netherlands.
Abstract:
Herein, we report the first fully automated continuous-flow platform for fluorescence quenching studies and Stern-Volmer analysis. All the components of the platform were automated and controlled by a self-written Python script. A user-friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern-Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies.
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