Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.6K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.6K
Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response01:15

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

371
Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
371
Pulse rhythm01:30

Pulse rhythm

1.4K
Pulse rhythm refers to the pattern of pulsations within specific intervals, offering valuable insights into the regularity or irregularity of the heart's beats as observed through the pattern of pulsation within specific intervals. A regular pulse exhibits a consistent heart rate with uniform waveforms and pulsation force, variations of which can be classified as normal, weak, or bounding.
Conversely, an irregular pulse pattern is termed dysrhythmia, stemming from disruptions in cardiac...
1.4K
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

41.7K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
41.7K
Disturbances in Heart Rhythm01:29

Disturbances in Heart Rhythm

3.0K
Arrhythmia or dysrhythmia refers to an abnormal heart rhythm caused by a defect in the heart's conduction system. It can cause the heart to beat irregularly, too quickly, or too slowly, leading to symptoms like chest pain, shortness of breath, and fainting. Factors such as stress, caffeine, alcohol, nicotine, cocaine, certain drugs, congenital defects, diseases, and electrolyte abnormalities can trigger arrhythmias.
Arrhythmias are categorized by their speed, rhythm, and origin. A slow heart...
3.0K
ECG Interpretation of Rhythms01:24

ECG Interpretation of Rhythms

14.2K
An electrocardiogram (ECG)graphically represents the heart's electrical activity on ECG paper or a monitor.
Components of the Electrocardiogram
The primary components of a normal ECG waveform in Normal sinus rhythm(NSR) include the P wave, PR interval, QRS complex, ST segment, T wave, and occasionally a U wave.
ECG waveforms are divided by vertical and horizontal lines at standard intervals.
The horizontal axis measures time and rate, and the vertical axis measures amplitude or voltage....
14.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cav3.1 is a neuronal leucine sensor that mediates satiety and weight loss in response to dietary protein.

Cell metabolism·2026
Same author

Adiponectin modulates the diurnal hepatic transcriptome and energy metabolism in male mice.

Endocrine connections·2026
Same author

The timing of antenatal glucocorticoids determines the receptor sensitivity in preterm infants.

The Journal of clinical endocrinology and metabolism·2026
Same author

Effects of Prednisolone Administration on Clock Gene Expression and Indices of Circadian Rhythms in Healthy Human Males.

The Journal of clinical endocrinology and metabolism·2025
Same author

Free water predicts dementia with Lewy bodies in isolated REM sleep behavior disorder.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Glucose-dependent insulinotropic polypeptide receptor signaling in oligodendrocytes increases the weight-loss action of GLP-1R agonism.

Cell metabolism·2025

Related Experiment Video

Updated: Feb 8, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

23.2K

Circadian clock rhythms in different adipose tissue model systems.

Maureen Friedrichs1, Isa Kolbe1, Julia Seemann1,2

  • 1a Center of Brain, Behavior & Metabolism , University of Lübeck , Lübeck , DE.

Chronobiology International
|July 12, 2018
PubMed
Summary
This summary is machine-generated.

Adipose tissue circadian clock models, including explants and cell cultures, effectively mimic in vivo rhythms. These systems are valuable for screening drugs targeting metabolic diseases.

Keywords:
adiposecircadian clockclock gene expressionexplants

More Related Videos

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

22.1K
Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila
09:09

Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila

Published on: January 13, 2014

8.5K

Related Experiment Videos

Last Updated: Feb 8, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

23.2K
Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Published on: January 24, 2013

22.1K
Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila
09:09

Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila

Published on: January 13, 2014

8.5K

Area of Science:

  • Metabolic research
  • Chronobiology
  • Adipose tissue biology

Background:

  • Circadian rhythms in adipose tissue regulate energy balance and are potential therapeutic targets for metabolic diseases.
  • Scalable adipose models are required to study these rhythms for drug discovery.

Purpose of the Study:

  • To compare molecular circadian clock regulation in various ex vivo and in vitro adipose tissue models.
  • To assess the suitability of these models for pharmacological screening.

Main Methods:

  • Utilized PER2::LUC reporter mice for explant cultures from different adipose depots.
  • Assessed luminescence rhythms in primary pre- and mature adipocytes.
  • Employed lentiviral transduction with Bmal1-luc and Per2-luc reporters in wild-type pre-adipocytes.
  • Analyzed core clock gene mRNA expression (Bmal1, Per2, Dbp, REV-erbα) in immortalized adipocytes.

Main Results:

  • Explant cultures showed stable luminescence rhythms across adipose depots.
  • Primary adipocytes exhibited stable rhythms, though mature adipocytes showed damping.
  • Lentiviral reporters confirmed stable circadian periods in pre-adipocytes.
  • Immortalized adipocytes displayed rhythmic clock gene expression with maturation-dependent increases in levels and amplitudes.
  • Observed phase differences in clock gene rhythms between in vivo and ex vivo conditions.

Conclusions:

  • Adipose culture systems largely recapitulate in vivo circadian clock regulation.
  • Both explant and cell-based models are suitable for large-scale screening of adipose clock regulators.
  • These models offer a promising avenue for identifying therapeutic targets for metabolic disorders.