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Related Experiment Video

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A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats

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Wireless Thermometry for Real-Time Temperature Recording on Thousand-Cell Level.

Can Li, Jianfei Sun, Qiwei Wang

IEEE Transactions on Bio-Medical Engineering

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A new wireless system enables real-time cellular temperature measurement in response to drugs. This high-throughput method accurately detects thermogenic responses in cell populations, aiding drug discovery.

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Area of Science:

Biomedical Engineering
Cell Biology
Sensor Technology

Background:

Accurate measurement of cellular temperature is crucial for understanding drug responses and cellular metabolism.
Existing methods may lack the real-time, high-throughput capabilities needed for dynamic cellular studies.

Purpose of the Study:

To develop a wireless, multichannel system for real-time measurement of cellular temperature responses to drugs.
To assess the system's accuracy, reliability, and suitability for studying cell population thermogenesis.

Main Methods:

A wireless, multichannel acquisition system utilizing a high-precision reference resistor and low intermittent current (≤0.14 mA) to minimize self-heating.
Culturing cells on a platinum thermal resistor surface within a small volume cell medium for measurement.
Employing software compensation for resistance deviations and validating sensor linearity (R² > 0.999) across 30°C-42°C.

Main Results:

Achieved a resistance resolution of 20 mΩ, corresponding to a temperature sensitivity of 0.01°C.
Confirmed good cell viability and growth on the non-toxic glass glaze sensor surface.
Observed a significant temperature rise in cell populations treated with norepinephrine, indicating drug-induced thermogenesis.

Conclusions:

The developed platinum sensor and multichannel system accurately determine temperature changes in cells.
This wireless, real-time, high-throughput method is ideal for evaluating the thermogenic capacity of cells interacting with other organisms or substances.