Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoreactive renin in human brain: distribution and properties.

K Mizuno, S Hashimoto, M Ojima

    Japanese Circulation Journal
    |September 1, 1985
    PubMed
    Summary

    Human brain tissue contains detectable levels of renin, an enzyme typically found in the kidneys. This discovery suggests the presence of the brain renin-angiotensin system in neuronal cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pregnancy outcomes in women with rheumatic diseases: a real-world observational study in Japan.

    Lupus·2019
    Same author

    Downregulation of miRNA-31 induces taxane resistance in ovarian cancer cells through increase of receptor tyrosine kinase MET.

    Oncogenesis·2013
    Same author

    Mutant p53 gain-of-function induces epithelial-mesenchymal transition through modulation of the miR-130b-ZEB1 axis.

    Oncogene·2012
    Same author

    Weekly paclitaxel/5-fluorouracil followed by platinum retreatment for patients with recurrent ovarian cancer: a single institution experience.

    European journal of gynaecological oncology·2009
    Same author

    Rapid detection of the cariogenic pathogens Streptococcus mutans and Streptococcus sobrinus using loop-mediated isothermal amplification.

    Oral microbiology and immunology·2007
    Same author

    Rapid detection of Actinobacillus actinomycetemcomitans using a loop-mediated isothermal amplification method.

    Oral microbiology and immunology·2007

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Endocrinology

    Background:

    • Renin is primarily known as a key enzyme in the kidney's renin-angiotensin system.
    • The presence and function of renin within the human brain have not been extensively characterized.

    Purpose of the Study:

    • To investigate the presence and biochemical characteristics of renin activity in human brain tissue.
    • To determine if brain renin shares properties with renal renin and to explore its potential endogenous synthesis.

    Main Methods:

    • Measurement of renin activity in various human brain regions.
    • Inhibition assays using specific antibodies against human renal renin.
    • Biochemical characterization including molecular weight, optimal pH, and electrofocusing.
    • Analysis of immunoreactive renin in neuroblastoma tissue.

    Related Experiment Videos

    Main Results:

    • Detectable renin activity was found in multiple brain regions, with the pineal gland and pituitary being the richest sources.
    • Brain renin exhibited some similarities to kidney renin (e.g., molecular weight, pH optimum, inactive form) but also distinct differences (e.g., electrofocusing pattern).
    • High activity of immunoreactive renin was detected in human neuroblastoma, suggesting the renin-angiotensin system's presence in neuronal cells.

    Conclusions:

    • The human brain possesses renin activity, localized in specific regions like the pineal and pituitary glands.
    • Biochemical evidence suggests both similarities and differences between brain and kidney renin, potentially indicating endogenous brain synthesis.
    • The findings support the existence of a functional renin-angiotensin system within human neuronal cells.