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Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
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Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila

Zaur M Kachaev1, Lyubov A Lebedeva1, Eugene N Kozlov1

  • 1a Laboratory of Gene Expression Regulation in Development , Institute of Gene Biology, Russian Academy of Sciences , Moscow , Russia.

Cell Cycle (Georgetown, Tex.)
|July 12, 2018
PubMed

Insights

Poly(A)-binding protein - interacting protein 2 (Paip2) is found in the nucleus, associating with active gene promoters. This RNA-dependent association suggests Paip2 is recruited to mRNAs during transcription.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cell Biology

Background:

  • Poly(A)-binding protein - interacting protein 2 (Paip2) is known to regulate mRNA translation and stability.
  • Paip2 was previously thought to be exclusively cytoplasmic.

Purpose of the Study:

  • To investigate the subcellular localization of Paip2.
  • To determine the function of nuclear Paip2 in gene regulation.

Main Methods:

  • Immunofluorescence microscopy to detect nuclear Paip2.
  • Chromatin immunoprecipitation (ChIP) to map Paip2 binding sites.
  • RNA depletion experiments to assess RNA dependence.

Main Results:

  • Paip2 is localized to the nucleus in various Drosophila tissues and cell types.
  • Nuclear Paip2 associates with chromatin at the promoter regions of active genes.
  • This chromatin association is indirect and dependent on RNA.

Conclusions:

  • Paip2 functions in the nucleus, in addition to its cytoplasmic roles.
  • Paip2 is recruited to nascent mRNAs co-transcriptionally.
  • This finding expands the known roles of translation factors in gene regulation.
Keywords:
Paip2mRNAnucleuspromotertranscription

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