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Genetic and Phylogenetic Characteristics of Pasteurella multocida Isolates From Different Host Species

Zhong Peng1,2,3, Wan Liang1,2,3,4, Fei Wang1,2,3

  • 1Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Frontiers in Microbiology
|July 13, 2018
PubMed

Insights

Pasteurella multocida strains from different hosts share no specific genes. Combining lipopolysaccharide (LPS) and multilocus sequence typing (MLST) genotypes better explains P. multocida strain relationships and phylogeny.

Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Bacterial Pathogenesis

Background:

  • Pasteurella multocida is a significant pathogen causing respiratory diseases across various animal species.
  • Understanding the genetic diversity of P. multocida strains is crucial for disease control and prevention.
  • Previous studies have characterized P. multocida based on limited genetic markers.

Purpose of the Study:

  • To investigate the genetic characteristics of Pasteurella multocida strains isolated from different host species.
  • To compare whole genome sequence (WGS) data of P. multocida from avian, bovine, porcine, and rabbit origins.
  • To determine if host-specific genes exist within P. multocida genomes.

Main Methods:

  • Whole genome sequencing (WGS) of P. multocida strains, including isolates from pigs.
  • Analysis of lipopolysaccharide (LPS) and multilocus sequence typing (MLST) genotypes.
  • Comparative genomic analysis to identify host-specific genes.
  • Phylogenetic analysis using whole-genome single nucleotide polymorphisms (SNPs) and core gene SNPs.

Main Results:

  • Predominant genotypes varied by host: avian (A:L1:ST129), bovine (B:L2:ST122 and A:L3:ST79), porcine (D:L6:ST50), and rabbit (A:L3:ST9).
  • No host-specific genes were identified in the P. multocida genome across the studied species.
  • Phylogenetic clustering strongly correlated with combined LPS and MLST genotypes, indicating their utility in explaining strain relationships.

Conclusions:

  • Pasteurella multocida lacks host-specific genes, suggesting broad adaptability.
  • The combination of LPS and MLST typing provides a robust framework for understanding P. multocida phylogeny and strain differentiation.
  • These findings enhance our understanding of P. multocida epidemiology and can inform targeted control strategies.
Keywords:
Pasteurella multocidacomparative genomics analysisdifferent host speciesgenetic characteristicsphylogeny

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