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Molecular detection of class 1, 2 and 3 integrons and some antimicrobial resistance genes in Salmonella Infantis

Fariba Asgharpour1, Seyed Mahmoud1,2,3, Amin Marashi2

  • 1Faculty of Para-Medicine, Babol University of Medical Sciences, Babol, Iran.

Iranian Journal of Microbiology
|July 13, 2018
PubMed
Abstract

Insights

Multidrug-resistant Salmonella strains are a global concern. This study found high frequencies of antimicrobial resistance genes and integrons in chicken broiler Salmonella isolates from Iran, highlighting the need for improved food safety management.

Area of Science:

  • Veterinary Microbiology
  • Food Safety
  • Antimicrobial Resistance

Background:

  • Multidrug-resistant (MDR) Salmonella strains pose a significant global health threat.
  • Antimicrobial resistance genes (ARGs) spread through various mechanisms, contributing to MDR strains.
  • Poultry, particularly chicken broilers, can act as a reservoir and vehicle for MDR Salmonella transmission.

Purpose of the Study:

  • To investigate the prevalence and distribution of class 1, 2, and 3 integrons in MDR Salmonella isolates.
  • To identify specific antimicrobial resistance genes conferring resistance to tetracyclines, chloramphenicol, and streptomycin.
  • To understand the genetic basis of multidrug resistance in Salmonella from Iranian chicken broilers.

Main Methods:

  • Polymerase Chain Reaction (PCR) assays were employed to detect specific ARGs (tetA, tetB, tetG, cat1, floR, strA).
  • Specific primers were used to identify the presence of integrase genes (intI1, intI2, intI3) for class 1, 2, and 3 integrons.
  • Isolates were sourced from chicken broilers in northern Iran.

Main Results:

  • Class 1, 2, and 3 integrons were detected in 36%, 42%, and 4% of MDR Salmonella isolates, respectively.
  • The tetA and tetB genes were highly prevalent (100% and 10.6%) among tetracycline-resistant isolates; tetG was not detected.
  • All chloramphenicol-resistant isolates harbored both cat1 and floR genes, and 18% of Salmonella Infantis isolates carried the strA gene.

Conclusions:

  • A high frequency of antimicrobial resistance genes and integrons was observed in Salmonella isolates from chicken broilers.
  • These findings underscore the importance of implementing effective management strategies to control food-borne diseases caused by MDR Salmonella.
  • Enhanced surveillance and control measures are crucial to mitigate the risk of MDR Salmonella transmission through the food supply chain.
Keywords:
IntegronsMultidrug resistancePoultrySalmonella infantis

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