Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

96.4K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
96.4K
Mitochondria01:37

Mitochondria

20.5K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
20.5K
5-Number Summary01:04

5-Number Summary

5.7K
In a dataset, the 5-number summary includes the minimum data value, the data value of the first quartile, the median data value or data value of the second quartile, the data value of the third quartile, and the maximum data value. These 5 data values can be visualized as a box and whisker plot.
In a box plot, the minimum and maximum data values represent the lower and upper whiskers in the graph, and the median is designated as the center of the box in the chart. The first quartile and third...
5.7K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

13.5K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
13.5K
Light Acquisition02:16

Light Acquisition

9.6K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
9.6K
Light as Energy01:35

Light as Energy

96.0K
The energy required to carry out photosynthesis is light— typically electromagnetic radiation from the sun. The range of all possible wavelengths is known as the electromagnetic spectrum.
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...
96.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

N-Phenyl indole derivatives as AT1 antagonists with anti-hypertension activities: Design, synthesis and biological evaluation.

European journal of medicinal chemistry·2016
Same author

Cefradine blocks solar-ultraviolet induced skin inflammation through direct inhibition of T-LAK cell-originated protein kinase.

Oncotarget·2016
Same author

Design, synthesis, cytotoxic activity and molecular docking studies of new 20(S)-sulfonylamidine camptothecin derivatives.

European journal of medicinal chemistry·2016
Same author

Label-free electrochemical immunosensor based on enhanced signal amplification between Au@Pd and CoFe2O4/graphene nanohybrid.

Scientific reports·2016
Same author

Recent Progress in Magnetic Resonance Imaging of the Embryonic and Neonatal Mouse Brain.

Frontiers in neuroanatomy·2016
Same author

Underuse of Primary Care in China: The Scale, Causes, and Solutions.

Journal of the American Board of Family Medicine : JABFM·2016

Related Experiment Video

Updated: Feb 7, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.6K

A pillar[5]arene-based [2]rotaxane lights up mitochondria.

Guocan Yu1, Dan Wu2, Yang Li2

  • 1State Key Laboratory of Chemical Engineering , Center for Chemistry of High-Performance & Novel Materials , Department of Chemistry , Zhejiang University , Hangzhou 310027 , P. R. China . Email: fhuang@zju.edu.cn ; ; Tel: +86-571-8795-3189.

Chemical Science
|July 13, 2018
PubMed
Summary

Researchers developed a novel supramolecular platform for targeted cancer therapy. This new system enables simultaneous organelle targeting, imaging, and drug delivery, overcoming limitations of traditional synthesis for advanced cancer treatments.

More Related Videos

Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars
08:02

Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars

Published on: February 11, 2020

9.4K
Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
07:19

Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars

Published on: August 20, 2014

12.7K

Related Experiment Videos

Last Updated: Feb 7, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.6K
Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars
08:02

Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars

Published on: February 11, 2020

9.4K
Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
07:19

Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars

Published on: August 20, 2014

12.7K

Area of Science:

  • Supramolecular Chemistry
  • Nanomedicine
  • Cancer Therapy

Background:

  • Developing single molecular platforms for simultaneous targeting, imaging, and treatment in cancer therapy is challenging.
  • Fluorescent probes with aggregation-induced emission (AIE) are valuable for tracking nanomedicines.
  • Mitochondria-targeting agents are crucial for precise cancer treatment.

Purpose of the Study:

  • To construct a pillar[5]arene-based [2]rotaxane with aggregation-induced emission (AIE) properties for mitochondria targeting.
  • To develop a prodrug system for simultaneous imaging and chemotherapy delivery.
  • To create a versatile drug delivery platform for various anticancer agents.

Main Methods:

  • Synthesized a pillar[5]arene-based [2]rotaxane (R1) using tetraphenylethene (TPE) and triphenylphosphonium (TPP) moieties.
  • Incorporated doxorubicin (DOX) into R1 to create a dual-fluorescence-quenched Förster resonance energy transfer (FRET) system (R2).
  • Conjugated other anticancer drugs with amine groups via imine formation to R1 for prodrug preparation.

Main Results:

  • R1 demonstrated enhanced AIE, high specificity for mitochondria, and superior photostability.
  • R2 functioned as a FRET system, with fluorescence recovery upon hydrolysis in endo/lysosomes.
  • Prodrugs released anticancer drugs via hydrolysis of pH-responsive imine bonds within cells.

Conclusions:

  • The developed supramolecular rotaxane platform offers a promising strategy for targeted cancer therapy.
  • This platform enables simultaneous mitochondria targeting, imaging, and drug delivery.
  • The system provides a versatile approach for developing advanced nanomedicines for cancer treatment.