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Yohimbine attenuates the delayed lethality induced in mice by amitraz, a formamidine pesticide
Abstract:
We have found that a single dose of amitraz, a formamidine pesticide, produces death in mice 2-5 days after dosing. To further examine this phenomenon, adult albino mice of both sexes were treated with either yohimbine (10 mg/kg, i.p.) or deionized water (6 ml/kg, i.p.), immediately before an injection of amitraz (600 mg/kg, i.p.) and twice daily thereafter for 8 days. Male mice treated with water were more susceptible than water-treated females to the lethal effects of amitraz. In addition, yohimbine treatment significantly decreased the number of deaths in both sexes. These data suggest that an alpha 2-adrenergic mechanism is involved in the delayed lethality produced by amitraz.
Insights
A single dose of the pesticide amitraz causes delayed death in mice. Yohimbine treatment reduced mortality, suggesting an alpha 2-adrenergic mechanism is involved in amitraz toxicity.
Area of Science:
- Toxicology
- Pharmacology
- Pesticide research
Background:
- Amitraz, a formamidine pesticide, is known to cause adverse effects.
- Delayed mortality following amitraz exposure has been observed in rodent models.
Purpose of the Study:
- To investigate the mechanism underlying the delayed lethality of amitraz in mice.
- To determine the role of the alpha 2-adrenergic system in amitraz-induced toxicity.
Main Methods:
- Adult male and female albino mice were administered amitraz (600 mg/kg, i.p.).
- Mice were co-treated with yohimbine (10 mg/kg, i.p.) or deionized water.
- Mortality rates and sex-specific differences in susceptibility were assessed over 8 days.
Main Results:
- Amitraz administration resulted in delayed death in mice, occurring 2-5 days post-dosing.
- Male mice exhibited higher susceptibility to amitraz lethality compared to females.
- Co-administration of yohimbine significantly reduced the number of deaths in both sexes.
Conclusions:
- The findings indicate that an alpha 2-adrenergic mechanism is implicated in the delayed lethality caused by amitraz.
- Yohimbine's protective effect highlights the potential involvement of adrenergic pathways in amitraz toxicity.
- Further research into alpha 2-adrenergic receptor interactions may elucidate novel therapeutic strategies for pesticide poisoning.