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In vivo 13C-MRI using SAMBADENA.

Andreas B Schmidt1,2, Stephan Berner1,3,4, Moritz Braig1

  • 1Medical Physics, Department of Radiology, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Plos One
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Summary

Researchers developed a new, low-cost method called SAMBADENA to create hyperpolarized tracers for faster, more sensitive MRI scans. This breakthrough enables real-time metabolic imaging in vivo, overcoming previous limitations of MRI.

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Area of Science:

  • Biomedical Imaging
  • Magnetic Resonance Imaging (MRI)
  • Hyperpolarization Techniques

Background:

  • Magnetic Resonance Imaging (MRI) has limited sensitivity for in vivo metabolic detection.
  • Hyperpolarization (HP) significantly enhances MRI signal but is typically slow, costly, and requires specialized equipment.
  • Existing HP methods are a barrier to widespread clinical adoption for metabolic imaging.

Purpose of the Study:

  • To introduce and validate a novel, cost-effective HP method (SAMBADENA) for in vivo MRI.
  • To demonstrate the feasibility of producing HP tracers within the MRI system.
  • To enable fast, sensitive metabolic imaging applications previously inaccessible with conventional MRI.

Main Methods:

  • Developed Synthesis Amid the Magnet Bore Allows Dramatically Enhanced Nuclear Alignment (SAMBADENA), a parahydrogen induced polarization (PHIP) method.
  • Implemented a setup for rapid, in-situ HP tracer production directly within the MR scanner.
  • Performed in vivo 13C-MRI angiography in an adult mouse using a SAMBADENA-generated tracer.

Main Results:

  • Successfully produced a hyperpolarized angiography tracer within seconds using SAMBADENA.
  • Achieved first-ever in vivo 13C-angiography in a mouse, visualizing major blood vessels.
  • Demonstrated HP of P13C > 20% for tracer solutions, indicating high polarization efficiency.

Conclusions:

  • SAMBADENA offers a simple, low-cost, and fast alternative for producing hyperpolarized MRI tracers.
  • This method significantly enhances MRI sensitivity, enabling previously challenging in vivo metabolic imaging.
  • SAMBADENA has the potential to unlock the full capabilities of MRI for clinical diagnostics and research.