Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

Photoreceptors and Plant Responses to Light

Photoreceptors and Plant Responses to Light

Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.

Scatter Plot

Scatter Plot

The most common and easiest way to display the relationship between two variables, x and y, is a scatter plot. A scatter plot shows the direction of a relationship between the variables. A clear direction happens when there is either:

Design Example: Maintaining Level of an Embankment

Design Example: Maintaining Level of an Embankment

Constructing a roadway embankment over uneven terrain requires precise leveling to ensure stability and proper drainage. Surveyors use a leveling instrument and staff to calculate ground elevations and determine the required fill material at each point along the embankment alignment.The process begins by positioning a leveling instrument near a benchmark with a known elevation. A backsight reading establishes the instrument height, which serves as a reference for subsequent measurements. A...

The Wave Nature of Light

The Wave Nature of Light

The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms and was able to demonstrate that white light consists of the individual colors of the rainbow combined together. Newton explained his optics findings in terms of a "corpuscular" view of light, in which light was composed of streams of extremely tiny particles traveling at high speeds according to Newton's laws of motion.

Role of Vitamins in Maintaining Bone Health

Role of Vitamins in Maintaining Bone Health

The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Beyond healing: rethinking traditional lateral internal sphincterotomy in the long term.

Techniques in coloproctology·2026

Same author

From prediction to clinical action in colorectal surgery: rethinking DLNN use for postoperative risk.

Techniques in coloproctology·2025

Same author

Surgical Management of Chronic Subdural Hematoma: A Single Center Experience.

Nigerian journal of clinical practice·2025

Same author

Correction to: Does onlay mesh placement in emergency laparotomy prevent incisional hernia? A prospective randomized double‑blind study.

Hernia : the journal of hernias and abdominal wall surgery·2025

Same author

Morphometric Analysis of the Tibial Tunnel after Primary and Revision Anterior Cruciate Ligament Reconstruction.

Malaysian orthopaedic journal·2025

Same author

Prevalence of rectus diastasis is higher in patients with inguinal hernia.

Hernia : the journal of hernias and abdominal wall surgery·2023

Search research articles

Related Experiment Video

Updated: Feb 7, 2026

Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes

Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes

Published on: July 7, 2023

Characterization of bevacizumab by dynamic light scattering while maintaining its native structure.

S Akbas, A Sahin, S Calis

|July 14, 2018

Summary

Bevacizumab protein aggregation was studied using dynamic light scattering (DLS) without dilution. This method successfully determined aggregation onset, aiding biosimilar development for bevacizumab formulations.

More Related Videos

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Published on: June 20, 2019

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Related Experiment Videos

Last Updated: Feb 7, 2026

Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes

Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes

Published on: July 7, 2023

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Published on: June 20, 2019

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Area of Science:

Biopharmaceutical formulation
Protein characterization
Analytical chemistry

Background:

Bevacizumab (Avastin®) patents are expiring, increasing interest in biosimilar development.
Protein aggregation is a critical challenge in formulating monoclonal antibody drugs like bevacizumab.
Dynamic light scattering (DLS) is a suitable technique for analyzing protein size and aggregation without sample dilution.

Purpose of the Study:

To investigate the hydrodynamic dimensions, aggregates, and aggregation onset of bevacizumab using DLS.
To maintain the native structure of bevacizumab during DLS analysis.
To assess the utility of DLS for characterizing commercial bevacizumab formulations and supporting biosimilar development.

Main Methods:

Characterization of bevacizumab (Altuzan®) using dynamic light scattering (DLS).
Evaluation of intensity, volume, and number-based particle size distribution profiles.
Determination of aggregation onset by monitoring changes with increasing temperature.

Main Results:

Hydrodynamic dimensions and aggregation profiles of bevacizumab were successfully investigated.
The aggregation onset of the bevacizumab formulation was determined against increasing temperature.
Native protein structure was preserved during DLS measurements.

Conclusions:

Preserving the native structure of commercial bevacizumab formulations in DLS measurements is feasible.
DLS analysis of native bevacizumab formulations provides valuable insights for product characterization.
This approach supports the development and characterization of bevacizumab biosimilars.