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Updated: Feb 7, 2026
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Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes
Published on: July 7, 2023
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Characterization of bevacizumab by dynamic light scattering while maintaining its native structure.
Die Pharmazie
|July 14, 2018
Summary
Bevacizumab protein aggregation was studied using dynamic light scattering (DLS) without dilution. This method successfully determined aggregation onset, aiding biosimilar development for bevacizumab formulations.
Area of Science:
- Biopharmaceutical formulation
- Protein characterization
- Analytical chemistry
Background:
- Bevacizumab (Avastin®) patents are expiring, increasing interest in biosimilar development.
- Protein aggregation is a critical challenge in formulating monoclonal antibody drugs like bevacizumab.
- Dynamic light scattering (DLS) is a suitable technique for analyzing protein size and aggregation without sample dilution.
Purpose of the Study:
- To investigate the hydrodynamic dimensions, aggregates, and aggregation onset of bevacizumab using DLS.
- To maintain the native structure of bevacizumab during DLS analysis.
- To assess the utility of DLS for characterizing commercial bevacizumab formulations and supporting biosimilar development.
Main Methods:
- Characterization of bevacizumab (Altuzan®) using dynamic light scattering (DLS).
- Evaluation of intensity, volume, and number-based particle size distribution profiles.
- Determination of aggregation onset by monitoring changes with increasing temperature.
Main Results:
- Hydrodynamic dimensions and aggregation profiles of bevacizumab were successfully investigated.
- The aggregation onset of the bevacizumab formulation was determined against increasing temperature.
- Native protein structure was preserved during DLS measurements.
Conclusions:
- Preserving the native structure of commercial bevacizumab formulations in DLS measurements is feasible.
- DLS analysis of native bevacizumab formulations provides valuable insights for product characterization.
- This approach supports the development and characterization of bevacizumab biosimilars.
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