Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

769
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
769
Instrumentation Amplifier01:25

Instrumentation Amplifier

1.1K
An electrocardiography (ECG) machine is an essential piece of medical equipment used to monitor the electrical activity of the heart. It operates by detecting small electrical changes on the skin that result from the depolarization of the heart muscle during each heartbeat. However, these signals are in the microvolt range and can be easily overwhelmed by noise or interference.
To overcome this challenge, an ECG machine utilizes an instrumentation amplifier. This specialized amplifier is...
1.1K
Instrument Transformers01:23

Instrument Transformers

495
Instrument transformers, comprising voltage transformers (VTs) and current transformers (CTs), play crucial roles in power substations by providing isolated replicas of current or voltage for measurement and protection purposes. Voltage transformers reduce the primary voltage to levels suitable for relay operation and measurement, while current transformers scale down the primary current. The primary winding of a current transformer often consists of a single turn, achieved by threading the...
495
Uncertainty in Measurement: Reading Instruments02:46

Uncertainty in Measurement: Reading Instruments

53.2K
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
53.2K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.2K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.2K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.6K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Contrary Effects of Cholesterol on Single-Component and Synaptic Vesicle Mimicking Lipid Bilayers.

The journal of physical chemistry letters·2025
Same author

Access, utilisation and barriers to lymphedema care: A mixed method study in Puducherry, India.

Acta tropica·2025
Same author

Molecules in the Serotonin-Melatonin Synthesis Pathway Have Distinct Interactions with Lipid Membranes.

The journal of physical chemistry. B·2025
Same author

Protein Silencing with Self-Peptides.

The journal of physical chemistry. B·2025
Same author

Effects of a Serotonergic Psychedelic on the Lipid Bilayer.

ACS chemical neuroscience·2024
Same author

Chronic chemogenetic activation of hippocampal progenitors enhances adult neurogenesis and modulates anxiety-like behavior and fear extinction learning.

IBRO neuroscience reports·2024

Related Experiment Video

Updated: Feb 7, 2026

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.8K

Single-molecule photobleaching: Instrumentation and applications.

Simli Dey1, Sudipta Maiti

  • 1Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India.

Journal of Biosciences
|July 14, 2018
PubMed
Summary

Single-molecule photobleaching (smPB) precisely measures protein oligomer size distribution, aiding disease research. This method, applied to Amylin oligomers linked to diabetes, offers advantages over average-size techniques.

More Related Videos

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.3K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.8K

Related Experiment Videos

Last Updated: Feb 7, 2026

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.8K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.3K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.8K

Area of Science:

  • Biophysics
  • Molecular Biology
  • Biochemistry

Background:

  • Protein aggregation is implicated in various incurable diseases.
  • Accurate oligomer size distribution measurement is crucial for understanding aggregation.
  • Existing methods like FCS provide average sizes but lack resolution for distributions.

Purpose of the Study:

  • To detail the single-molecule photobleaching (smPB) method using a home-built total internal reflection fluorescence (TIRF) microscope.
  • To demonstrate the application of smPB for characterizing protein oligomer size distribution.
  • To compare smPB with fluorescence correlation spectroscopy (FCS) for analyzing Amylin (hIAPP) oligomers.

Main Methods:

  • Construction of a home-built total internal reflection fluorescence (TIRF) microscope.
  • Implementation of the single-molecule photobleaching (smPB) technique.
  • Characterization of Amylin (hIAPP) oligomer solutions.

Main Results:

  • The smPB technique was successfully implemented on the custom TIRF microscope.
  • smPB provided a direct measure of the number of monomers per oligomeric particle.
  • The study generated an oligomer size distribution for Amylin (hIAPP) in solution.

Conclusions:

  • smPB is a powerful tool for characterizing molecular assemblies and their size distributions.
  • The technique offers advantages over methods providing only average sizes, particularly for protein aggregation studies.
  • smPB provides valuable insights into the aggregation of Amylin (hIAPP), relevant to Type II diabetes research.