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Laser Micromachining for Polymer Surface Topography Design

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Published on: September 19, 2025

Designed Surface Topographies Control ICAM-1 Expression in Tonsil-Derived Human Stromal Cells.

Aliaksei S Vasilevich¹, Frédéric Mourcin², Anouk Mentink¹

¹Laboratory for Cell Biology-Inspired Tissue Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Netherlands.

Frontiers in Bioengineering and Biotechnology

|July 14, 2018

Summary

Researchers used surface topographies to differentiate stromal cell precursors into Fibroblastic Reticular Cells (FRCs). This method overcomes limitations in FRC availability and function for improved immune response studies.

Keywords:

ICAM-1 fibroblastic reticular cells lymph node mechanobiology surface topography

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Area of Science:

Immunology
Cell Biology
Biomaterials Science

Background:

Fibroblastic reticular cells (FRCs) are crucial stromal cells in lymph nodes, supporting immune cell adhesion and communication.
Limited availability and functional decline of FRCs in culture impede research on immune responses.
Mechanotransduction offers a potential method to generate mature FRCs from precursor cells.

Purpose of the Study:

To investigate the use of designed surface topographies for inducing FRC differentiation from tonsil-derived stromal cells (TSCs).
To identify specific surface features that promote or inhibit FRC differentiation markers.
To establish a method for generating functional FRCs for immunological research.

Main Methods:

Utilized a TopoChip with 2176 distinct surface topographies to culture undifferentiated TSCs.
Monitored cell morphology and ICAM-1 expression (a key FRC differentiation marker) using image analysis and machine learning.
Validated findings on ICAM-1 expression and FRC differentiation using flow cytometry.

Main Results:

Identified 112 topographies that upregulated ICAM-1 expression and 72 that downregulated it.
Discovered significant correlations between cell shape, surface topography design, and ICAM-1 expression levels.
Confirmed that TSCs are mechanosensitive and respond to topographical cues.

Conclusions:

Designed surface topographies can effectively induce FRC differentiation from TSCs without requiring chemical differentiation factors.
Specific topographical features can be leveraged to control FRC differentiation.
This approach provides a promising strategy to overcome limitations in FRC availability and function for immunological studies.