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Khat Induced Toxicity: Role on Its Modulating Effects on Inflammation and Oxidative Stability
Siddig Ibrahim Abdelwahab1, Rashad Alsanosy1, Manal Mohamed Elhassan Taha1,2
1Substance Abuse Research Centre, Jazan University, Jazan, Saudi Arabia.
Biomed Research International
|July 14, 2018
Summary
Long-term khat chewing causes cell damage and oxidative stress due to its polyphenolic compounds. This study reveals khat
Area of Science:
- Pharmacology
- Biochemistry
- Cell Biology
Background:
- Long-term khat (Catha edulis) chewing is linked to adverse health effects.
- Khat constituents may disrupt cellular equilibrium, leading to oxidative damage.
- Understanding khat's impact on cellular processes is crucial.
Purpose of the Study:
- To investigate the role of khat in cellular toxicity, oxidative stability, and inflammation.
- To analyze the antioxidant properties of khat extract.
- To assess khat's effects on macrophage cell lines.
Main Methods:
- Khat was extracted using 60% methanol.
- Phenolic and flavonoid content were quantified.
- Antioxidant capacity was measured using DPPH, ORAC, and FRAP assays.
- Cellular inflammation, oxidative stability, and viability were assessed in RAW 264.7 cells.
Main Results:
- Khat extract is rich in polyphenols and flavonoids.
- Khat demonstrated significant antioxidant potency in DPPH, ORAC, and FRAP assays.
- Khat reduced nitric oxide production and inhibited reactive oxygen species.
- Khat induced cytotoxicity in RAW 264.7 cells.
Conclusions:
- Khat possesses substantial antioxidant properties.
- Its polyphenolic constituents may contribute to both beneficial antioxidant effects and harmful cytotoxicity.
- Heavy khat consumption may lead to toxicity due to these compounds.
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