Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.8K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.8K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.4K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.4K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

976
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
976
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

5.2K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
5.2K
NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

11.2K
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
11.2K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

2.4K
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Raman Spectroscopy and Imaging Reveal the Effect of β-Carotene Supplementation on Brain Cancer Cells.

Biochemistry·2025
Same author

Modifications of Cytochrome <i>c</i> by Retinoic Acid Play a Crucial Role in Mitochondrial Dysfunction of Triple-Positive Human Breast Cancer Cells: Raman Spectroscopy and Imaging Study.

ACS omega·2025
Same author

Characteristics and Outcomes of Pulmonary Embolism Patients Transferred After Activation of Pulmonary Embolism Response Team and Admitted from Local Emergency Department.

Journal of clinical medicine·2025
Same author

A Novel HER2 Protein Identification Methodology in Breast Cancer Cells Using Raman Spectroscopy and Raman Imaging: An Analytical Validation Study.

Journal of medicinal chemistry·2024
Same author

Metabolism changes caused by glucose in normal and cancer human brain cell lines by Raman imaging and chemometric methods.

Scientific reports·2024
Same author

Monitoring alterations of all-<i>trans</i>-retinal in human brain cancer cells by label-free confocal Raman imaging: regulation of the redox status of cytochrome <i>c</i>.

RSC advances·2024

Related Experiment Video

Updated: Feb 7, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.8K

Raman spectroscopy for medulloblastoma.

Bartosz Polis1, Anna Imiela2, Lech Polis3

  • 1Department of Neurosurgery and Neurotraumatology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska St., 93-338, Lodz, Poland. jezza@post.pl.

Child'S Nervous System : Chns : Official Journal of the International Society for Pediatric Neurosurgery
|July 14, 2018
PubMed
Summary

Raman spectroscopy identifies medulloblastoma by analyzing tissue biochemistry. This technique differentiates cancerous tissue, rich in protein and low in lipids, from healthy tissue.

Keywords:
Embryonal tumorMedulloblastomaRamanSpectroscopy

More Related Videos

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems
07:44

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

15.6K
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.6K

Related Experiment Videos

Last Updated: Feb 7, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.8K
Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems
07:44

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

15.6K
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.6K

Area of Science:

  • Biochemistry
  • Spectroscopy
  • Oncology

Background:

  • Medulloblastoma is a common pediatric brain tumor.
  • Accurate tissue differentiation is crucial for diagnosis and treatment.
  • Current diagnostic methods may require further refinement.

Purpose of the Study:

  • To analyze the biochemical composition of medulloblastoma and normal central nervous system (CNS) tissues using Raman spectroscopy.
  • To identify specific Raman biomarkers for differentiating tumorous from normal tissues.

Main Methods:

  • Confocal Raman microscopy (WITec alpha 300 RSA) was used to generate Raman images.
  • Tissue samples included medulloblastoma (grade IV) and normal tissues from the safety margin.
  • Analysis focused on biochemical differences, particularly protein and lipid content.

Main Results:

  • Raman vibrational signatures successfully predicted tumorous biochemistry.
  • The study identified medulloblastoma based on its distinct biochemical profile.
  • Tumorous tissue showed higher protein and lower lipid content compared to normal tissue.

Conclusions:

  • Raman spectroscopy (RS) can effectively discriminate between normal and medulloblastoma tissues.
  • The technique offers a powerful tool for monitoring tissue morphology and biochemistry.
  • RS has the potential to enhance medulloblastoma diagnostics.