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Proper splicing contributes to visual function in the aging Drosophila eye.

Rachel Stegeman1, Hana Hall1, Spencer E Escobedo1

  • 1Department of Biochemistry, Purdue University, West Lafayette, Indiana.

Aging Cell
|July 14, 2018
PubMed
Summary
This summary is machine-generated.

Aging eyes show altered gene splicing, impacting visual function. Decreased splicing factors contribute to visual decline and circular RNA buildup in older flies, suggesting a link between splicing and aging.

Keywords:
Drosophilaagingeyephotoreceptorsplicingvision

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Area of Science:

  • Molecular Biology
  • Genetics
  • Neuroscience
  • Aging Research

Background:

  • Splicing patterns change with age, affecting the transcriptome.
  • Age-related decline in visual behavior in Drosophila correlates with reduced expression of splicing factors in photoreceptors.

Purpose of the Study:

  • To investigate the role of age-regulated splicing-associated genes in splicing and visual function.
  • To determine if proper splicing is essential for visual behavior.

Main Methods:

  • Identified differential splicing events in eyes and photoreceptors of young and old Drosophila.
  • Knocked down age-regulated splicing factors in young flies' photoreceptors to assess effects on phototaxis and splicing.
  • Analyzed changes in alternative splicing and circular RNA accumulation.

Main Results:

  • Aging photoreceptors exhibit differential splicing of numerous visual function genes.
  • Aging eyes show increased accumulation of circular RNAs, indicative of noncanonical splicing.
  • Knockdown of age-regulated splicing factors in young flies impaired visual behavior and altered splicing patterns, including increased circular RNA accumulation.

Conclusions:

  • Decreased expression of splicing factors contributes to age-related differential splicing and circular RNA accumulation in photoreceptors.
  • These splicing alterations are linked to defective visual behavior during aging.