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Related Experiment Video

Updated: Feb 7, 2026

Bergmeyer Glucose Quantification for Microbiological Samples

Bergmeyer Glucose Quantification for Microbiological Samples

Published on: January 17, 2025

Stratiform Protein Microtube Reactors Containing Glucose Oxidase Layer.

Ryo Adachi¹, Motofusa Akiyama¹, Yoshitsugu Morita¹

¹Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8555, Japan.

Chemistry, an Asian Journal

|July 14, 2018

Summary

Researchers created protein microtubes with glucose oxidase (GOD) for efficient glucose oxidation. The enzyme

Keywords:

enzymes layer-by-layer assembly microreactors multilayered structure proteins

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Hydrogen Production and Utilization in a Membrane Reactor

Hydrogen Production and Utilization in a Membrane Reactor

Published on: March 10, 2023

Area of Science:

Biomaterials Science
Enzyme Engineering
Nanotechnology

Background:

Protein-based microreactors offer unique platforms for biocatalysis.
Layer-by-layer assembly is a versatile technique for fabricating complex nanostructures.

Purpose of the Study:

To synthesize and characterize stratiform protein microtubes containing glucose oxidase (GOD).
To investigate the catalytic efficiency of GOD-functionalized microtubes for glucose oxidation.
To elucidate the impact of GOD layer positioning on catalytic activity.

Main Methods:

Layer-by-layer assembly using human serum albumin (HSA), poly(l-arginine) (PLA), and GOD on a polycarbonate membrane template.
Scanning Electron Microscopy (SEM) for structural characterization.
Enzyme kinetic studies to determine Michaelis constant (K M) and catalytic turnover rate (k cat).

Main Results:

Uniform hollow protein microtubes with controlled dimensions (1.17 μm outer diameter, 135 nm wall thickness) were successfully fabricated.
The synthesized microtube reactors exhibited high catalytic efficiency in β-d-glucose oxidation.
Catalytic activity varied depending on the strategic placement of the glucose oxidase layer within the microtube wall.

Conclusions:

Stratiform protein microtubes are effective platforms for immobilizing enzymes like GOD.
The positioning of the enzyme layer significantly influences the overall catalytic performance.
This study provides insights into designing advanced enzyme microreactors for biotechnological applications.