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Related Experiment Video

Updated: Feb 7, 2026

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence
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Ratiometric Two-Photon Fluorescent Probe for Detecting and Imaging Hypochlorite.

Yen Leng Pak1, Sang Jun Park2, Qingling Xu3

  • 1Department of Chemistry and Nano Science , Ewha Womans University , Seoul 120-750 , Korea.

Analytical Chemistry
|July 14, 2018
PubMed
Summary

A new fluorescent probe, B6S, can detect hypochlorite (OCl-) with high specificity. This probe is suitable for imaging OCl- in living cells and tissues, aiding research into its biological roles.

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Area of Science:

  • Chemical Biology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Hypochlorite (OCl-) is a reactive species involved in various biological processes.
  • Developing specific and sensitive probes for OCl- detection is crucial for understanding its physiological and pathological roles.
  • Existing detection methods may lack specificity or require harsh conditions.

Purpose of the Study:

  • To develop a ratiometric fluorescent probe, B6S, for the selective detection of hypochlorite.
  • To evaluate the probe's performance under biological conditions and its suitability for live-cell imaging.

Main Methods:

  • Synthesis of the B6S probe incorporating pyrene and imidazoline-2-thione.
  • Spectroscopic analysis to determine probe characteristics and selectivity.
  • Cytotoxicity assays and two-photon microscopy for live-cell and tissue imaging.

Main Results:

  • B6S demonstrated high specificity for hypochlorite over other reactive oxygen and nitrogen species.
  • The probe exhibited a low detection limit and functioned effectively under physiological conditions.
  • Low cytotoxicity of B6S allowed for successful OCl- imaging in living cells and tissues via two-photon microscopy.

Conclusions:

  • B6S is a sensitive and selective ratiometric fluorescent probe for hypochlorite detection.
  • The probe's properties make it a valuable tool for exploring the biological functions of hypochlorite in vivo.
  • B6S facilitates advanced imaging of hypochlorite in biological systems using two-photon microscopy.